Molecular Biology

  1. Molecular Biology Techniques
    1. Basic Techniques
      1. DNA Extraction and Purification
        1. Cell Lysis
          1. Protein Removal
            1. DNA Precipitation
              1. Quality Assessment
              2. RNA Extraction
                1. RNase-free Conditions
                  1. RNA Integrity
                  2. Gel Electrophoresis
                    1. Agarose Gels
                      1. Polyacrylamide Gels
                        1. DNA/RNA Visualization
                        2. Spectrophotometry
                          1. Nucleic Acid Quantification
                            1. Purity Assessment
                          2. Restriction Enzymes
                            1. Enzyme Types
                              1. Recognition Sequences
                                1. Cleavage Patterns
                                  1. Restriction Mapping
                                  2. Cloning and Recombinant DNA
                                    1. Vectors
                                      1. Plasmid Vectors
                                        1. Bacteriophage Vectors
                                          1. Cosmid Vectors
                                            1. BAC and YAC Vectors
                                            2. Cloning Process
                                              1. Insert Preparation
                                                1. Vector Preparation
                                                  1. Ligation
                                                    1. Transformation
                                                      1. Selection and Screening
                                                      2. Expression Systems
                                                        1. Bacterial Expression
                                                          1. Eukaryotic Expression
                                                            1. Cell-free Systems
                                                          2. Polymerase Chain Reaction
                                                            1. PCR Components
                                                              1. Template DNA
                                                                1. Primers
                                                                  1. DNA Polymerase
                                                                    1. dNTPs
                                                                    2. PCR Cycling
                                                                      1. Denaturation
                                                                        1. Annealing
                                                                          1. Extension
                                                                          2. PCR Variations
                                                                            1. Reverse Transcription PCR
                                                                              1. Quantitative PCR
                                                                                1. Multiplex PCR
                                                                                  1. Long-range PCR
                                                                                2. DNA Sequencing
                                                                                  1. Sanger Sequencing
                                                                                    1. Chain Termination Method
                                                                                      1. Sequencing Reactions
                                                                                        1. Data Analysis
                                                                                        2. Next-generation Sequencing
                                                                                          1. Illumina Sequencing
                                                                                            1. Ion Torrent
                                                                                              1. 454 Pyrosequencing
                                                                                                1. Library Preparation
                                                                                                  1. Data Processing
                                                                                                  2. Third-generation Sequencing
                                                                                                    1. PacBio SMRT
                                                                                                      1. Oxford Nanopore
                                                                                                        1. Single-molecule Sequencing
                                                                                                      2. Gene Expression Analysis
                                                                                                        1. Northern Blotting
                                                                                                          1. In Situ Hybridization
                                                                                                            1. Microarrays
                                                                                                              1. RNA Sequencing
                                                                                                                1. Quantitative RT-PCR
                                                                                                                2. Protein Analysis
                                                                                                                  1. SDS-PAGE
                                                                                                                    1. Western Blotting
                                                                                                                      1. Immunoprecipitation
                                                                                                                        1. Mass Spectrometry
                                                                                                                          1. Protein Crystallography
                                                                                                                            1. NMR Spectroscopy

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                                                                                                                          12. Advanced Topics