Biocatalysis

  1. Enzyme Engineering
    1. Rational Design
      1. Structure-Function Relationships
        1. Site-Directed Mutagenesis
          1. Computational Modeling
            1. Molecular Docking
            2. Directed Evolution
              1. Random Mutagenesis
                1. Error-Prone PCR
                  1. Chemical Mutagenesis
                  2. DNA Recombination
                    1. DNA Shuffling
                      1. StEP
                        1. RACHITT
                        2. Selection and Screening
                          1. Iterative Improvement
                          2. Semi-Rational Approaches
                            1. Focused Libraries
                              1. Saturation Mutagenesis
                                1. CASTing
                                2. Engineering Targets
                                  1. Activity Enhancement
                                    1. Stability Improvement
                                      1. Substrate Specificity
                                        1. pH Tolerance
                                          1. Temperature Resistance
                                            1. Solvent Tolerance

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                                          7. Biocatalyst Discovery and Development

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                                          9. Reaction Media and Conditions