Biocatalysis

  1. Enzyme Kinetics
    1. Basic Kinetic Principles
      1. Reaction Rate Theory
        1. Activation Energy
          1. Catalytic Rate Enhancement
          2. Michaelis-Menten Kinetics
            1. Model Assumptions
              1. Derivation
                1. Kinetic Parameters
                  1. Vmax
                    1. Km
                      1. kcat
                        1. kcat/Km
                      2. Kinetic Analysis Methods
                        1. Lineweaver-Burk Plot
                          1. Hanes-Woolf Plot
                            1. Eadie-Hofstee Plot
                              1. Direct Fitting Methods
                              2. Enzyme Inhibition
                                1. Reversible Inhibition
                                  1. Competitive Inhibition
                                    1. Noncompetitive Inhibition
                                      1. Uncompetitive Inhibition
                                        1. Mixed Inhibition
                                        2. Irreversible Inhibition
                                          1. Covalent Modification
                                            1. Suicide Inhibition
                                          2. Complex Kinetics
                                            1. Multi-substrate Reactions
                                              1. Allosteric Enzymes
                                                1. Cooperative Binding
                                                  1. Product Inhibition
                                                  2. Environmental Effects
                                                    1. Temperature Dependence
                                                      1. pH Effects
                                                        1. Ionic Strength
                                                          1. Solvent Effects

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                                                        4. Enzyme Catalysis Mechanisms

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                                                        6. Whole Cell Biocatalysis