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Engineering
Biochemical and Bioprocess Engineering
Biocatalysis
1. Introduction to Biocatalysis
2. Enzyme Structure and Function
3. Enzyme Classification and Nomenclature
4. Enzyme Catalysis Mechanisms
5. Enzyme Kinetics
6. Whole Cell Biocatalysis
7. Biocatalyst Discovery and Development
8. Enzyme Engineering
9. Reaction Media and Conditions
10. Enzyme Immobilization
11. Bioreactor Design and Operation
12. Downstream Processing
13. Industrial Applications
14. Advanced Biocatalytic Systems
15. Emerging Technologies
16. Economic and Regulatory Aspects
17. Future Perspectives
6.
Whole Cell Biocatalysis
6.1.
Advantages of Whole Cells
6.1.1.
Cofactor Regeneration
6.1.2.
Enzyme Protection
6.1.3.
Multi-step Reactions
6.1.4.
Cost Effectiveness
6.2.
Types of Cell Systems
6.2.1.
Growing Cells
6.2.2.
Resting Cells
6.2.3.
Permeabilized Cells
6.2.4.
Immobilized Cells
6.2.5.
Spores
6.3.
Cell Preparation Methods
6.3.1.
Cell Harvesting
6.3.2.
Washing Procedures
6.3.3.
Permeabilization Techniques
6.3.4.
Storage Methods
6.4.
Challenges and Limitations
6.4.1.
Mass Transfer Barriers
6.4.2.
Side Reactions
6.4.3.
Cell Viability
6.4.4.
Product Toxicity
6.4.5.
Genetic Stability
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5. Enzyme Kinetics
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7. Biocatalyst Discovery and Development