Biocatalysis

  1. Whole Cell Biocatalysis
    1. Advantages of Whole Cells
      1. Cofactor Regeneration
        1. Enzyme Protection
          1. Multi-step Reactions
            1. Cost Effectiveness
            2. Types of Cell Systems
              1. Growing Cells
                1. Resting Cells
                  1. Permeabilized Cells
                    1. Immobilized Cells
                      1. Spores
                      2. Cell Preparation Methods
                        1. Cell Harvesting
                          1. Washing Procedures
                            1. Permeabilization Techniques
                              1. Storage Methods
                              2. Challenges and Limitations
                                1. Mass Transfer Barriers
                                  1. Side Reactions
                                    1. Cell Viability
                                      1. Product Toxicity
                                        1. Genetic Stability

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                                      5. Enzyme Kinetics

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                                      7. Biocatalyst Discovery and Development