Biocatalysis

  1. Biocatalyst Discovery and Development
    1. Natural Sources
      1. Microbial Diversity
        1. Bacteria
          1. Archaea
            1. Fungi
              1. Yeasts
              2. Plant Enzymes
                1. Animal Enzymes
                  1. Marine Organisms
                    1. Extremophiles
                    2. Screening Strategies
                      1. Activity-Based Screening
                        1. High-Throughput Methods
                          1. Colorimetric Assays
                            1. Fluorogenic Substrates
                            2. Metagenomics
                              1. Environmental DNA Libraries
                                1. Functional Screening
                                  1. Sequence-Based Approaches
                                    1. Cultivation-Independent Methods
                                    2. Recombinant Production
                                      1. Gene Cloning
                                        1. Expression Systems
                                          1. Escherichia coli
                                            1. Pichia pastoris
                                              1. Saccharomyces cerevisiae
                                                1. Bacillus subtilis
                                                  1. Mammalian Cells
                                                  2. Protein Purification
                                                    1. Cell Disruption
                                                      1. Chromatographic Methods
                                                        1. Refolding Techniques

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                                                    6. Whole Cell Biocatalysis

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                                                    8. Enzyme Engineering