Microbiology

  1. Biotechnology and Recombinant DNA
    1. Molecular Tools
      1. Restriction Enzymes
        1. Recognition Sequences
          1. Cleavage Patterns
            1. Sticky and Blunt Ends
            2. DNA Ligase
              1. Joining DNA Fragments
                1. Recombinant Molecule Formation
                2. Cloning Vectors
                  1. Plasmid Vectors
                    1. Bacteriophage Vectors
                      1. Cosmid Vectors
                        1. Artificial Chromosomes
                        2. Polymerase Chain Reaction
                          1. PCR Components
                            1. Thermal Cycling
                              1. DNA Amplification
                                1. PCR Applications
                              2. Genetic Engineering Techniques
                                1. Recombinant DNA Construction
                                  1. Gene Isolation
                                    1. Vector Preparation
                                      1. Ligation Reactions
                                      2. DNA Introduction Methods
                                        1. Transformation
                                          1. Electroporation
                                            1. Microinjection
                                              1. Biolistics
                                              2. Clone Selection
                                                1. Antibiotic Resistance Markers
                                                  1. Blue-White Screening
                                                    1. Complementation
                                                    2. Gene Expression Systems
                                                      1. Prokaryotic Expression
                                                        1. Eukaryotic Expression
                                                          1. Protein Purification
                                                        2. Biotechnology Applications
                                                          1. Medical Applications
                                                            1. Therapeutic Proteins
                                                              1. Human Insulin
                                                                1. Growth Hormone
                                                                  1. Interferons
                                                                  2. Vaccines
                                                                    1. Subunit Vaccines
                                                                      1. DNA Vaccines
                                                                      2. Gene Therapy
                                                                        1. Vector Systems
                                                                          1. Therapeutic Targets
                                                                          2. Diagnostic Applications
                                                                            1. DNA Probes
                                                                              1. PCR Diagnostics
                                                                            2. Agricultural Applications
                                                                              1. Transgenic Crops
                                                                                1. Pest Resistance
                                                                                  1. Herbicide Tolerance
                                                                                    1. Nutritional Enhancement
                                                                                    2. Industrial Applications
                                                                                      1. Enzyme Production
                                                                                        1. Biofuel Production
                                                                                          1. Bioremediation
                                                                                            1. Pharmaceutical Manufacturing
                                                                                            2. Research Applications
                                                                                              1. DNA Sequencing
                                                                                                1. Genome Projects
                                                                                                  1. Functional Genomics
                                                                                                    1. Proteomics

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                                                                                                7. Microbial Genetics

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                                                                                                9. Microbial Classification and Identification