Microbiology

  1. Microbial Growth
    1. Growth Requirements
      1. Physical Factors
        1. Temperature Requirements
          1. Psychrophiles
            1. Psychrotrophs
              1. Mesophiles
                1. Thermophiles
                  1. Hyperthermophiles
                  2. pH Requirements
                    1. Acidophiles
                      1. Neutrophiles
                        1. Alkaliphiles
                          1. pH Tolerance Ranges
                          2. Osmotic Pressure
                            1. Isotonic Conditions
                              1. Hypotonic Stress
                                1. Hypertonic Adaptation
                                  1. Halophiles
                                    1. Osmotolerant Organisms
                                    2. Hydrostatic Pressure
                                      1. Atmospheric Pressure
                                        1. Barophiles
                                          1. Deep-Sea Adaptations
                                        2. Chemical Requirements
                                          1. Carbon Sources
                                            1. Organic Carbon
                                              1. Inorganic Carbon
                                                1. Carbon Assimilation
                                                2. Nitrogen Sources
                                                  1. Organic Nitrogen
                                                    1. Inorganic Nitrogen
                                                      1. Nitrogen Fixation
                                                      2. Sulfur and Phosphorus
                                                        1. Sulfur Sources
                                                          1. Phosphorus Sources
                                                            1. Metabolic Roles
                                                            2. Trace Elements
                                                              1. Metal Cofactors
                                                                1. Enzyme Functions
                                                                  1. Micronutrient Requirements
                                                                  2. Oxygen Requirements
                                                                    1. Obligate Aerobes
                                                                      1. Facultative Anaerobes
                                                                        1. Obligate Anaerobes
                                                                          1. Aerotolerant Anaerobes
                                                                            1. Microaerophiles
                                                                            2. Growth Factors
                                                                              1. Vitamins
                                                                                1. Amino Acids
                                                                                  1. Nucleotides
                                                                                    1. Auxotrophs
                                                                                2. Biofilm Formation
                                                                                  1. Biofilm Development Stages
                                                                                    1. Initial Attachment
                                                                                      1. Microcolony Formation
                                                                                        1. Maturation
                                                                                          1. Dispersal
                                                                                          2. Biofilm Structure
                                                                                            1. Extracellular Matrix
                                                                                              1. Water Channels
                                                                                                1. Cell Communication
                                                                                                2. Medical Significance
                                                                                                  1. Device-Associated Infections
                                                                                                    1. Antibiotic Resistance
                                                                                                      1. Chronic Infections
                                                                                                      2. Industrial Significance
                                                                                                        1. Biofouling
                                                                                                          1. Corrosion
                                                                                                            1. Water Treatment
                                                                                                          2. Culture Media
                                                                                                            1. Media Classification
                                                                                                              1. Chemically Defined Media
                                                                                                                1. Known Composition
                                                                                                                  1. Synthetic Media
                                                                                                                  2. Complex Media
                                                                                                                    1. Unknown Composition
                                                                                                                      1. Natural Ingredients
                                                                                                                      2. Selective Media
                                                                                                                        1. Growth Inhibition
                                                                                                                          1. Differential Selection
                                                                                                                          2. Differential Media
                                                                                                                            1. Metabolic Indicators
                                                                                                                              1. Colony Characteristics
                                                                                                                              2. Enrichment Media
                                                                                                                                1. Nutrient Enhancement
                                                                                                                                  1. Selective Enrichment
                                                                                                                                2. Anaerobic Culture Methods
                                                                                                                                  1. Oxygen Removal
                                                                                                                                    1. Anaerobic Chambers
                                                                                                                                      1. Gas Pak Systems
                                                                                                                                        1. Reducing Agents
                                                                                                                                      2. Pure Culture Techniques
                                                                                                                                        1. Isolation Methods
                                                                                                                                          1. Streak Plate Method
                                                                                                                                            1. Pour Plate Method
                                                                                                                                              1. Spread Plate Method
                                                                                                                                                1. Serial Dilution
                                                                                                                                                2. Culture Maintenance
                                                                                                                                                  1. Stock Cultures
                                                                                                                                                    1. Preservation Methods
                                                                                                                                                      1. Subculturing
                                                                                                                                                    2. Growth Patterns
                                                                                                                                                      1. Binary Fission
                                                                                                                                                        1. DNA Replication
                                                                                                                                                          1. Cell Division
                                                                                                                                                            1. Septum Formation
                                                                                                                                                            2. Generation Time
                                                                                                                                                              1. Doubling Time
                                                                                                                                                                1. Species Variation
                                                                                                                                                                  1. Environmental Effects
                                                                                                                                                                  2. Growth Curve Phases
                                                                                                                                                                    1. Lag Phase
                                                                                                                                                                      1. Metabolic Adjustment
                                                                                                                                                                        1. Enzyme Synthesis
                                                                                                                                                                        2. Exponential Phase
                                                                                                                                                                          1. Logarithmic Growth
                                                                                                                                                                            1. Maximum Growth Rate
                                                                                                                                                                            2. Stationary Phase
                                                                                                                                                                              1. Growth Limitation
                                                                                                                                                                                1. Death-Growth Balance
                                                                                                                                                                                2. Death Phase
                                                                                                                                                                                  1. Cell Lysis
                                                                                                                                                                                    1. Population Decline
                                                                                                                                                                                3. Growth Measurement
                                                                                                                                                                                  1. Direct Methods
                                                                                                                                                                                    1. Plate Counts
                                                                                                                                                                                      1. Colony Forming Units
                                                                                                                                                                                        1. Dilution Plating
                                                                                                                                                                                        2. Membrane Filtration
                                                                                                                                                                                          1. Water Analysis
                                                                                                                                                                                            1. Low-Density Samples
                                                                                                                                                                                            2. Most Probable Number
                                                                                                                                                                                              1. Statistical Estimation
                                                                                                                                                                                                1. Liquid Media
                                                                                                                                                                                                2. Direct Microscopic Count
                                                                                                                                                                                                  1. Cell Counting
                                                                                                                                                                                                    1. Viability Questions
                                                                                                                                                                                                  2. Indirect Methods
                                                                                                                                                                                                    1. Turbidity Measurement
                                                                                                                                                                                                      1. Spectrophotometry
                                                                                                                                                                                                        1. Optical Density
                                                                                                                                                                                                        2. Metabolic Activity
                                                                                                                                                                                                          1. Oxygen Consumption
                                                                                                                                                                                                            1. Acid Production
                                                                                                                                                                                                              1. Enzyme Activity
                                                                                                                                                                                                              2. Dry Weight Determination
                                                                                                                                                                                                                1. Biomass Measurement
                                                                                                                                                                                                                  1. Cell Mass

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                                                                                                                                                                                                            4. Microbial Metabolism

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                                                                                                                                                                                                            6. Microbial Growth Control