Biophysical Chemistry

  1. Spectroscopic Methods
    1. Electromagnetic Radiation
      1. Wave-Particle Duality
        1. Energy-Wavelength Relationships
          1. Interaction with Matter
            1. Selection Rules
            2. Absorption Spectroscopy
              1. Beer-Lambert Law
                1. Absorbance Measurements
                  1. Extinction Coefficients
                    1. Path Length Effects
                    2. UV-Visible Spectroscopy
                      1. Electronic Transitions
                        1. Chromophore Identification
                          1. Protein Concentration Determination
                            1. Nucleic Acid Quantification
                            2. Infrared Spectroscopy
                              1. Vibrational Modes
                                1. Functional Group Analysis
                                  1. Protein Secondary Structure
                                    1. Hydrogen Bonding Studies
                                  2. Fluorescence Spectroscopy
                                    1. Fluorescence Principles
                                      1. Excitation and Emission
                                        1. Quantum Yield
                                          1. Fluorescence Lifetime
                                          2. Intrinsic Fluorescence
                                            1. Tryptophan Fluorescence
                                              1. Tyrosine Fluorescence
                                                1. Environmental Sensitivity
                                                2. Extrinsic Fluorophores
                                                  1. Fluorescent Dyes
                                                    1. Labeling Strategies
                                                      1. FRET Applications
                                                      2. Quenching Mechanisms
                                                        1. Static Quenching
                                                          1. Dynamic Quenching
                                                            1. Stern-Volmer Analysis
                                                          2. Circular Dichroism
                                                            1. Optical Activity
                                                              1. Circularly Polarized Light
                                                                1. Protein Secondary Structure Analysis
                                                                  1. Nucleic Acid Conformation Studies
                                                                    1. Thermal Denaturation Monitoring
                                                                    2. Raman Spectroscopy
                                                                      1. Scattering Principles
                                                                        1. Vibrational Analysis
                                                                          1. Resonance Raman
                                                                            1. Surface-Enhanced Raman

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                                                                          7. Macromolecular Structure

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                                                                          9. Structural Determination Methods