Bioreactor Design and Operation

  1. Modes of Bioreactor Operation
    1. Batch Operation
      1. Process Characteristics
        1. Closed System Operation
          1. Time-Dependent Profiles
            1. Batch Cycle Components
            2. Operational Phases
              1. Preparation Phase
                1. Inoculation Phase
                  1. Growth Phase
                    1. Production Phase
                      1. Harvesting Phase
                      2. Advantages and Limitations
                        1. Simplicity of Operation
                          1. Contamination Risk
                            1. Productivity Limitations
                              1. Product Quality Control
                              2. Applications
                                1. High-Value Products
                                  1. Research and Development
                                    1. Small-Scale Production
                                  2. Fed-Batch Operation
                                    1. Operating Principles
                                      1. Semi-Continuous Operation
                                        1. Substrate Addition Strategies
                                          1. Volume Increase Management
                                          2. Feeding Strategies
                                            1. Constant Feed Rate
                                              1. Exponential Feeding
                                                1. Linear Feeding
                                                  1. Feedback-Controlled Feeding
                                                    1. Multi-Stage Feeding
                                                    2. Control Parameters
                                                      1. Feed Rate Control
                                                        1. Nutrient Concentration Control
                                                          1. pH Control During Feeding
                                                            1. Dissolved Oxygen Control
                                                            2. Applications
                                                              1. High Cell Density Cultures
                                                                1. Recombinant Protein Production
                                                                  1. Antibiotic Production
                                                                    1. Enzyme Production
                                                                    2. Optimization Strategies
                                                                      1. Feed Profile Optimization
                                                                        1. Substrate Limitation Control
                                                                          1. Product Inhibition Management
                                                                        2. Continuous Operation
                                                                          1. Chemostat Operation
                                                                            1. Steady-State Principles
                                                                              1. Dilution Rate Control
                                                                                1. Material Balance Equations
                                                                                  1. Washout Conditions
                                                                                  2. Turbidostat Operation
                                                                                    1. Biomass Concentration Control
                                                                                      1. Optical Density Feedback
                                                                                        1. Growth Rate Optimization
                                                                                        2. Perfusion Systems
                                                                                          1. Cell Retention Methods
                                                                                            1. Microfiltration
                                                                                              1. Ultrafiltration
                                                                                                1. Centrifugal Separation
                                                                                                  1. Acoustic Separation
                                                                                                  2. Media Exchange Strategies
                                                                                                    1. Perfusion Rate Control
                                                                                                      1. Bleed Stream Management
                                                                                                      2. Multi-Stage Systems
                                                                                                        1. Series Configuration
                                                                                                          1. Parallel Configuration
                                                                                                            1. Recycle Streams
                                                                                                            2. Applications
                                                                                                              1. Large-Scale Production
                                                                                                                1. Waste Treatment
                                                                                                                  1. Research Studies
                                                                                                                    1. Continuous Manufacturing

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                                                                                                                6. Instrumentation, Measurement, and Control

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                                                                                                                8. Scale-Up and Scale-Down