Genetics and Genomics

  1. Applied Genetics and Genomics
    1. Recombinant DNA Technology
      1. Restriction Enzyme Systems
        1. Type I Enzymes
          1. Type II Enzymes
            1. Type III Enzymes
              1. Recognition Sequences
                1. Cutting Patterns
                  1. Star Activity
                  2. Cloning Vectors
                    1. Plasmid Vectors
                      1. pUC Series
                        1. pBR322
                          1. Expression Vectors
                          2. Bacteriophage Vectors
                            1. Lambda Vectors
                              1. M13 Vectors
                              2. Cosmid Vectors
                                1. Artificial Chromosomes
                                  1. YACs
                                    1. BACs
                                      1. PACs
                                    2. DNA Amplification
                                      1. Polymerase Chain Reaction
                                        1. Denaturation Step
                                          1. Annealing Step
                                            1. Extension Step
                                              1. Primer Design
                                                1. Thermostable Polymerases
                                                2. PCR Variations
                                                  1. Reverse Transcription PCR
                                                    1. Quantitative PCR
                                                      1. Nested PCR
                                                        1. Multiplex PCR
                                                      2. DNA Analysis Techniques
                                                        1. Gel Electrophoresis
                                                          1. Agarose Gels
                                                            1. Polyacrylamide Gels
                                                              1. Pulsed-Field Electrophoresis
                                                              2. Southern Blotting
                                                                1. Northern Blotting
                                                                  1. Western Blotting
                                                                    1. DNA Sequencing Methods
                                                                  2. Genetic Engineering Applications
                                                                    1. Genetically Modified Organisms
                                                                      1. Transformation Methods
                                                                        1. Chemical Transformation
                                                                          1. Electroporation
                                                                            1. Microinjection
                                                                              1. Biolistic Transformation
                                                                              2. Selection Systems
                                                                                1. Antibiotic Resistance
                                                                                  1. Nutritional Selection
                                                                                    1. Reporter Genes
                                                                                  2. Agricultural Applications
                                                                                    1. Herbicide Resistance
                                                                                      1. Insect Resistance
                                                                                        1. Disease Resistance
                                                                                          1. Nutritional Enhancement
                                                                                            1. Stress Tolerance
                                                                                            2. Industrial Applications
                                                                                              1. Recombinant Proteins
                                                                                                1. Enzyme Production
                                                                                                  1. Biofuel Production
                                                                                                    1. Pharmaceutical Manufacturing
                                                                                                    2. Environmental Applications
                                                                                                      1. Bioremediation
                                                                                                        1. Biosensors
                                                                                                          1. Waste Treatment
                                                                                                        2. Advanced Gene Editing
                                                                                                          1. CRISPR-Cas Systems
                                                                                                            1. Cas9 Mechanism
                                                                                                              1. Guide RNA Design
                                                                                                                1. PAM Sequence Recognition
                                                                                                                  1. DNA Cleavage
                                                                                                                  2. Cas12 Systems
                                                                                                                    1. Cas13 Systems
                                                                                                                      1. Base Editing
                                                                                                                        1. Prime Editing
                                                                                                                        2. Other Editing Systems
                                                                                                                          1. Zinc Finger Nucleases
                                                                                                                            1. TALENs
                                                                                                                              1. Meganucleases
                                                                                                                              2. Delivery Methods
                                                                                                                                1. Viral Vectors
                                                                                                                                  1. Lipofection
                                                                                                                                    1. Electroporation
                                                                                                                                      1. Microinjection
                                                                                                                                    2. Medical Genetics and Genomics
                                                                                                                                      1. Genetic Testing
                                                                                                                                        1. Diagnostic Testing
                                                                                                                                          1. Predictive Testing
                                                                                                                                            1. Carrier Testing
                                                                                                                                              1. Pharmacogenetic Testing
                                                                                                                                                1. Prenatal Testing
                                                                                                                                                  1. Amniocentesis
                                                                                                                                                    1. Chorionic Villus Sampling
                                                                                                                                                      1. Non-Invasive Prenatal Testing
                                                                                                                                                      2. Newborn Screening
                                                                                                                                                      3. Genetic Counseling
                                                                                                                                                        1. Risk Assessment
                                                                                                                                                          1. Family History Analysis
                                                                                                                                                            1. Test Result Interpretation
                                                                                                                                                              1. Psychosocial Support
                                                                                                                                                                1. Reproductive Options
                                                                                                                                                                2. Gene Therapy
                                                                                                                                                                  1. Somatic Gene Therapy
                                                                                                                                                                    1. Ex Vivo Approaches
                                                                                                                                                                      1. In Vivo Delivery
                                                                                                                                                                        1. Vector Systems
                                                                                                                                                                        2. Germline Gene Therapy
                                                                                                                                                                          1. Clinical Trial Phases
                                                                                                                                                                            1. Safety Considerations
                                                                                                                                                                            2. Pharmacogenomics
                                                                                                                                                                              1. Drug Metabolism Genes
                                                                                                                                                                                1. Drug Response Prediction
                                                                                                                                                                                  1. Adverse Reaction Prevention
                                                                                                                                                                                    1. Personalized Dosing
                                                                                                                                                                                  2. Cancer Genetics
                                                                                                                                                                                    1. Oncogene Function
                                                                                                                                                                                      1. Growth Factor Genes
                                                                                                                                                                                        1. Growth Factor Receptor Genes
                                                                                                                                                                                          1. Signal Transduction Genes
                                                                                                                                                                                            1. Transcription Factor Genes
                                                                                                                                                                                            2. Tumor Suppressor Genes
                                                                                                                                                                                              1. p53 Function
                                                                                                                                                                                                1. Rb Function
                                                                                                                                                                                                  1. BRCA1 and BRCA2
                                                                                                                                                                                                    1. APC Gene
                                                                                                                                                                                                    2. Cancer Development Models
                                                                                                                                                                                                      1. Two-Hit Hypothesis
                                                                                                                                                                                                        1. Multi-Step Carcinogenesis
                                                                                                                                                                                                          1. Clonal Evolution
                                                                                                                                                                                                          2. Hereditary Cancer Syndromes
                                                                                                                                                                                                            1. Familial Adenomatous Polyposis
                                                                                                                                                                                                              1. Hereditary Breast and Ovarian Cancer
                                                                                                                                                                                                                1. Lynch Syndrome
                                                                                                                                                                                                                  1. Li-Fraumeni Syndrome
                                                                                                                                                                                                                2. Forensic Genetics
                                                                                                                                                                                                                  1. DNA Profiling
                                                                                                                                                                                                                    1. CODIS System
                                                                                                                                                                                                                      1. STR Analysis
                                                                                                                                                                                                                        1. Y-Chromosome Analysis
                                                                                                                                                                                                                          1. Mitochondrial DNA Analysis
                                                                                                                                                                                                                          2. Paternity Testing
                                                                                                                                                                                                                            1. Mass Disaster Victim Identification
                                                                                                                                                                                                                              1. Cold Case Investigations
                                                                                                                                                                                                                                1. Database Searches

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                                                                                                                                                                                                                              8. Genomics and Bioinformatics

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                                                                                                                                                                                                                              1. Introduction to Genetics and Heredity