Clinical Microbiology

  1. Laboratory Safety and Quality Management
    1. Biosafety Principles and Practices
      1. Standard Precautions
        1. Hand Hygiene
          1. When to Perform
            1. Proper Technique
              1. Hand Sanitizers vs Soap
              2. Safe Handling of Specimens
                1. Primary Barriers
                  1. Secondary Containment
                  2. Sharps Safety
                    1. Safe Disposal
                      1. Needlestick Prevention
                    2. Universal Precautions
                      1. Blood and Body Fluid Precautions
                        1. Application in Microbiology
                        2. Biosafety Levels
                          1. BSL-1 Requirements
                            1. Organisms and Practices
                              1. Facility Requirements
                              2. BSL-2 Requirements
                                1. Organisms and Practices
                                  1. Facility and Equipment Requirements
                                  2. BSL-3 Requirements
                                    1. High-Risk Organisms
                                      1. Specialized Facilities
                                      2. BSL-4 Requirements
                                        1. Maximum Containment
                                          1. Specialized Training
                                        2. Personal Protective Equipment
                                          1. Types of PPE
                                            1. Gloves
                                              1. Laboratory Coats
                                                1. Eye Protection
                                                  1. Respiratory Protection
                                                  2. Selection Criteria
                                                    1. Proper Use and Removal
                                                      1. Disposal and Decontamination
                                                      2. Laboratory Design and Engineering Controls
                                                        1. Ventilation Systems
                                                          1. Biological Safety Cabinets
                                                            1. Class I Cabinets
                                                              1. Class II Cabinets
                                                                1. Class III Cabinets
                                                                2. Chemical Fume Hoods
                                                                3. Decontamination and Sterilization
                                                                  1. Disinfection Methods
                                                                    1. Chemical Disinfectants
                                                                      1. UV Irradiation
                                                                        1. Heat Disinfection
                                                                        2. Sterilization Techniques
                                                                          1. Autoclaving
                                                                            1. Dry Heat Sterilization
                                                                              1. Gas Sterilization
                                                                                1. Filtration
                                                                                2. Monitoring Sterilization Efficacy
                                                                                  1. Biological Indicators
                                                                                    1. Chemical Indicators
                                                                                  2. Waste Management
                                                                                    1. Classification of Laboratory Waste
                                                                                      1. Biohazardous Waste
                                                                                        1. Sharps Waste
                                                                                          1. Chemical Waste
                                                                                          2. Treatment and Disposal Methods
                                                                                            1. Regulatory Requirements
                                                                                          3. Quality Assurance and Quality Control
                                                                                            1. Total Quality Management
                                                                                              1. Pre-analytical Phase
                                                                                                1. Test Ordering
                                                                                                  1. Appropriate Test Selection
                                                                                                    1. Clinical Correlation
                                                                                                    2. Specimen Collection
                                                                                                      1. Collection Procedures
                                                                                                        1. Patient Preparation
                                                                                                          1. Timing of Collection
                                                                                                          2. Specimen Labeling
                                                                                                            1. Patient Identification
                                                                                                              1. Specimen Type
                                                                                                                1. Collection Time
                                                                                                              2. Analytical Phase
                                                                                                                1. Test Performance
                                                                                                                  1. Standard Operating Procedures
                                                                                                                    1. Method Validation
                                                                                                                    2. Instrument Maintenance
                                                                                                                      1. Preventive Maintenance
                                                                                                                        1. Calibration
                                                                                                                          1. Performance Verification
                                                                                                                          2. Quality Control Testing
                                                                                                                            1. Daily QC
                                                                                                                              1. Control Materials
                                                                                                                                1. Control Limits
                                                                                                                              2. Post-analytical Phase
                                                                                                                                1. Result Verification
                                                                                                                                  1. Critical Values
                                                                                                                                    1. Delta Checks
                                                                                                                                    2. Result Reporting
                                                                                                                                      1. Turnaround Time
                                                                                                                                        1. Report Format
                                                                                                                                        2. Data Management
                                                                                                                                          1. Record Keeping
                                                                                                                                            1. Data Security
                                                                                                                                        3. Specimen Management
                                                                                                                                          1. Specimen Collection Guidelines
                                                                                                                                            1. Collection Techniques by Specimen Type
                                                                                                                                              1. Blood Cultures
                                                                                                                                                1. Urine Specimens
                                                                                                                                                  1. Respiratory Specimens
                                                                                                                                                    1. Wound Specimens
                                                                                                                                                      1. Cerebrospinal Fluid
                                                                                                                                                      2. Patient Preparation
                                                                                                                                                        1. Collection Timing
                                                                                                                                                        2. Specimen Transport and Storage
                                                                                                                                                          1. Transport Media
                                                                                                                                                            1. Bacterial Transport Media
                                                                                                                                                              1. Viral Transport Media
                                                                                                                                                                1. Anaerobic Transport
                                                                                                                                                                2. Temperature Requirements
                                                                                                                                                                  1. Room Temperature
                                                                                                                                                                    1. Refrigerated
                                                                                                                                                                      1. Frozen
                                                                                                                                                                      2. Time Limits for Processing
                                                                                                                                                                      3. Specimen Processing
                                                                                                                                                                        1. Accessioning
                                                                                                                                                                          1. Preliminary Examination
                                                                                                                                                                            1. Specimen Preparation
                                                                                                                                                                            2. Specimen Rejection Criteria
                                                                                                                                                                              1. Inadequate Volume
                                                                                                                                                                                1. Improper Collection
                                                                                                                                                                                  1. Contamination
                                                                                                                                                                                    1. Delayed Transport
                                                                                                                                                                                      1. Communication with Clinicians
                                                                                                                                                                                    2. Quality Assessment Programs
                                                                                                                                                                                      1. Internal Quality Control
                                                                                                                                                                                        1. Control Strains
                                                                                                                                                                                          1. Control Charts
                                                                                                                                                                                            1. Corrective Actions
                                                                                                                                                                                            2. External Quality Assessment
                                                                                                                                                                                              1. Proficiency Testing Programs
                                                                                                                                                                                                1. Performance Evaluation
                                                                                                                                                                                                  1. Corrective Actions
                                                                                                                                                                                                  2. Laboratory Accreditation
                                                                                                                                                                                                    1. Accreditation Bodies
                                                                                                                                                                                                      1. Standards and Requirements
                                                                                                                                                                                                        1. Inspection Process
                                                                                                                                                                                                      2. Documentation and Record Keeping
                                                                                                                                                                                                        1. Standard Operating Procedures
                                                                                                                                                                                                          1. Quality Manual
                                                                                                                                                                                                            1. Training Records
                                                                                                                                                                                                              1. Incident Reporting

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                                                                                                                                                                                                          1. Introduction to Clinical Microbiology

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                                                                                                                                                                                                          3. Fundamental Laboratory Techniques