Plant Pathology

  1. Diagnosis of Plant Diseases
    1. Field Diagnosis
      1. Observation of Symptoms
        1. Necrosis
          1. Blights
            1. Leaf Spots
              1. Cankers
              2. Wilting
                1. Abnormal Growth
                  1. Galls
                    1. Stunting
                    2. Discoloration
                      1. Chlorosis
                        1. Mosaic Patterns
                      2. Observation of Signs of the Pathogen
                        1. Mycelium
                          1. Spores
                            1. Fruiting Bodies
                              1. Bacterial Ooze
                                1. Nematode Cysts
                                  1. Parasitic Plant Structures
                                  2. Use of Field Diagnostic Tools
                                  3. Laboratory Diagnostic Techniques
                                    1. Microscopy
                                      1. Light Microscopy
                                        1. Electron Microscopy
                                        2. Isolation and Culturing of Pathogens
                                          1. Media Preparation
                                            1. Pure Culture Techniques
                                            2. Serological Methods
                                              1. ELISA
                                                1. Lateral Flow Devices
                                                2. Molecular Methods
                                                  1. PCR
                                                    1. DNA Sequencing
                                                      1. DNA Barcoding
                                                        1. Real-Time PCR
                                                        2. Biochemical Tests
                                                        3. Koch's Postulates
                                                          1. Steps of Koch's Postulates
                                                            1. Application to Different Pathogens
                                                              1. Limitations and Modifications

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                                                            7. Plant Disease Epidemiology

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                                                            9. Principles of Plant Disease Management