Metabolic Engineering

  1. Host Organisms in Metabolic Engineering
    1. Prokaryotic Hosts
      1. Escherichia coli
        1. Genetic Tools Availability
          1. Fast Growth Rate
            1. Well-Characterized Metabolism
              1. Commonly Used Strains
                1. Limitations and Challenges
                2. Bacillus subtilis
                  1. Protein Secretion Capabilities
                    1. GRAS Status
                      1. Sporulation Properties
                        1. Genetic Manipulation Tools
                        2. Corynebacterium glutamicum
                          1. Amino Acid Production History
                            1. Industrial Robustness
                              1. Metabolic Characteristics
                              2. Pseudomonas putida
                                1. Solvent Tolerance
                                  1. Aromatic Compound Metabolism
                                    1. Environmental Applications
                                    2. Cyanobacteria
                                      1. Photosynthetic Capability
                                        1. CO2 Fixation
                                          1. Light-Driven Production
                                        2. Eukaryotic Hosts
                                          1. Saccharomyces cerevisiae
                                            1. Fermentation Capabilities
                                              1. GRAS Status
                                                1. Genetic Tools
                                                  1. Post-Translational Modifications
                                                  2. Pichia pastoris
                                                    1. Methanol Utilization
                                                      1. High-Level Protein Expression
                                                        1. Secretion Capabilities
                                                        2. Yarrowia lipolytica
                                                          1. Lipid Accumulation
                                                            1. Oleaginous Properties
                                                              1. Industrial Applications
                                                              2. Filamentous Fungi
                                                                1. Enzyme Production
                                                                  1. Secondary Metabolites
                                                                    1. Protein Secretion
                                                                    2. Mammalian Cells
                                                                      1. Complex Protein Folding
                                                                        1. Human-Like Glycosylation
                                                                          1. Biopharmaceutical Production
                                                                          2. Plant Systems
                                                                            1. Metabolic Engineering in Plants
                                                                              1. Transgenic Approaches
                                                                                1. Cell Culture Systems
                                                                              2. Alternative Systems
                                                                                1. Cell-Free Systems
                                                                                  1. In Vitro Protein Synthesis
                                                                                    1. Pathway Reconstitution
                                                                                      1. Advantages and Limitations
                                                                                      2. Synthetic Cells
                                                                                        1. Minimal Cell Approaches
                                                                                          1. Artificial Cell Construction

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                                                                                      6. Strategies for Pathway Optimization

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                                                                                      8. Applications and Case Studies