Metabolic Engineering

  1. Analytical Methods for Metabolic Engineering
    1. Quantifying Metabolites
      1. High-Performance Liquid Chromatography
        1. Principles of Separation
          1. Column Types
            1. Detection Methods
              1. Sample Preparation
              2. Gas Chromatography
                1. Sample Derivatization
                  1. Column Selection
                    1. Volatile Compound Analysis
                    2. Mass Spectrometry
                      1. Ionization Techniques
                        1. Mass Analyzers
                          1. Tandem MS
                            1. Data Interpretation
                            2. Nuclear Magnetic Resonance Spectroscopy
                              1. Principles of NMR
                                1. 1H NMR
                                  1. 13C NMR
                                    1. Metabolite Identification
                                  2. Multi-Omics Analysis
                                    1. Genomics
                                      1. Genome Sequencing Technologies
                                        1. Genome Assembly
                                          1. Annotation and Analysis
                                            1. Comparative Genomics
                                            2. Transcriptomics
                                              1. Microarray Technology
                                                1. RNA-Seq
                                                  1. Single-Cell RNA-Seq
                                                    1. Data Analysis Pipelines
                                                    2. Proteomics
                                                      1. Two-Dimensional PAGE
                                                        1. LC-MS/MS
                                                          1. Protein Quantification
                                                            1. Post-Translational Modifications
                                                            2. Metabolomics
                                                              1. Targeted Metabolomics
                                                                1. Untargeted Metabolomics
                                                                  1. Sample Preparation
                                                                    1. Data Processing and Analysis
                                                                    2. Fluxomics
                                                                      1. Metabolic Flux Measurement
                                                                        1. Integration with Other Omics
                                                                          1. Dynamic Flux Analysis
                                                                        2. Isotope Labeling Experiments
                                                                          1. 13C Metabolic Flux Analysis
                                                                            1. Experimental Design
                                                                              1. Tracer Selection
                                                                                1. Data Analysis and Modeling
                                                                                  1. Flux Calculation
                                                                                  2. Stationary MFA
                                                                                    1. Steady-State Assumptions
                                                                                      1. Mass Isotopomer Distribution
                                                                                      2. Non-Stationary MFA
                                                                                        1. Dynamic Labeling
                                                                                          1. Transient Analysis
                                                                                          2. Isotope Tracers
                                                                                            1. Carbon-13 Tracers
                                                                                              1. Nitrogen-15 Tracers
                                                                                                1. Deuterium Tracers
                                                                                                  1. Labeling Pattern Interpretation

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                                                                                              3. Tools for Genetic Modification

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                                                                                              5. Modeling and Analysis of Metabolic Networks