Human Genetics

  1. Genetic Technologies and Genomics
    1. DNA Sequencing Methods
      1. First-Generation Sequencing
        1. Sanger Method
          1. Chain Termination
            1. Capillary Electrophoresis
              1. Applications and Limitations
              2. Next-Generation Sequencing
                1. Sequencing Platforms
                  1. Library Preparation
                    1. Cluster Generation
                      1. Sequencing Chemistry
                        1. Data Analysis Pipelines
                        2. Third-Generation Sequencing
                          1. Single-Molecule Sequencing
                            1. Long-Read Technologies
                              1. Real-Time Sequencing
                                1. Applications and Advantages
                              2. Genomic Analysis Approaches
                                1. Whole Genome Sequencing
                                  1. Coverage Requirements
                                    1. Variant Calling
                                      1. Structural Variant Detection
                                        1. Clinical Applications
                                        2. Whole Exome Sequencing
                                          1. Exome Capture Methods
                                            1. Coverage Uniformity
                                              1. Variant Interpretation
                                                1. Diagnostic Yield
                                                2. Targeted Sequencing
                                                  1. Gene Panel Design
                                                    1. Amplicon Sequencing
                                                      1. Hybrid Capture
                                                        1. Clinical Validation
                                                      2. Molecular Cloning Techniques
                                                        1. Cloning Vectors
                                                          1. Plasmid Vectors
                                                            1. Viral Vectors
                                                              1. Artificial Chromosomes
                                                                1. Expression Systems
                                                                2. DNA Library Construction
                                                                  1. Genomic Libraries
                                                                    1. cDNA Libraries
                                                                      1. Library Screening
                                                                        1. Clone Characterization
                                                                        2. Polymerase Chain Reaction
                                                                          1. PCR Principles
                                                                            1. Primer Design
                                                                              1. PCR Variants
                                                                                1. Quantitative PCR
                                                                                  1. Clinical Applications
                                                                                2. Gene Editing Technologies
                                                                                  1. CRISPR-Cas Systems
                                                                                    1. Mechanism of Action
                                                                                      1. Guide RNA Design
                                                                                        1. Off-Target Effects
                                                                                          1. Delivery Methods
                                                                                            1. Therapeutic Applications
                                                                                            2. Other Editing Tools
                                                                                              1. Zinc Finger Nucleases
                                                                                                1. TAL Effector Nucleases
                                                                                                  1. Base Editors
                                                                                                    1. Prime Editors
                                                                                                    2. Genome Editing Applications
                                                                                                      1. Disease Modeling
                                                                                                        1. Therapeutic Development
                                                                                                          1. Agricultural Applications
                                                                                                            1. Ethical Considerations
                                                                                                          2. Functional Genomics
                                                                                                            1. Transcriptome Analysis
                                                                                                              1. RNA Sequencing
                                                                                                                1. Expression Profiling
                                                                                                                  1. Alternative Splicing Analysis
                                                                                                                    1. Single-Cell RNA-Seq
                                                                                                                    2. Proteome Analysis
                                                                                                                      1. Mass Spectrometry
                                                                                                                        1. Protein Identification
                                                                                                                          1. Quantitative Proteomics
                                                                                                                            1. Post-Translational Modifications
                                                                                                                            2. Epigenome Analysis
                                                                                                                              1. DNA Methylation Profiling
                                                                                                                                1. Histone Modification Mapping
                                                                                                                                  1. Chromatin Accessibility
                                                                                                                                    1. 3D Genome Organization

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                                                                                                                                10. Clinical Genetics and Genetic Medicine