Gene Editing

  1. Introduction to Gene Editing
    1. Core Principles of Targeted Genome Modification
      1. Definition and Scope of Gene Editing
        1. Precision vs. Random Modification
          1. Comparison with Traditional Genetic Engineering
            1. Recombinant DNA Technology
              1. Random Mutagenesis
                1. Selective Breeding
              2. Historical Context and Development
                1. Early Methods of Genetic Modification
                  1. Chemical Mutagenesis
                    1. Radiation-induced Mutations
                      1. Recombinant DNA Techniques
                      2. Evolution of Site-Specific Nucleases
                        1. First-generation Tools
                          1. Programmable Nucleases
                          2. Timeline of Gene Editing Milestones
                          3. Fundamental Concepts and Terminology
                            1. Nucleases
                              1. Endonucleases vs. Exonucleases
                                1. Site-specific vs. Non-specific
                                  1. Programmable Nucleases
                                  2. Double-Strand Breaks
                                    1. Importance in Gene Editing
                                      1. Cellular Response to DSBs
                                        1. Repair Pathway Activation
                                        2. DNA Repair Pathways in Gene Editing
                                          1. Homology-Directed Repair
                                            1. Template-dependent Repair
                                              1. Precise Editing Outcomes
                                              2. Non-Homologous End Joining
                                                1. Error-prone Repair
                                                  1. Knockout Applications
                                                  2. Alternative End Joining Pathways
                                                  3. Target Specificity
                                                    1. On-target vs. Off-target Effects
                                                      1. Specificity Determinants
                                                        1. Multiplexing Capabilities

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                                                    1. Foundational Concepts in Genetics

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                                                    3. Major Gene Editing Technologies