Electron microscopy techniques
Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization. In 2017, the Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution." Nature Methods also named cryo-EM as the "Method of the Year" in 2015. The Federal Institute of Technology, the University of Lausanne and the University of Geneva opened the Dubochet Center For Imaging (DCI) at the end of November 2021, in which the technology of Cryogenic electron microscopy is to be applied in the best possible way on the one hand, and further developed on the other. Less than a month after the first identification of the SARS-CoV-2 Omicron variant, researchers at the DCI were able to define its structure, identify the crucial mutations to circumvent individual vaccines and provide insights for new therapeutic approaches. (Wikipedia).
Chris Russo - Potential electron cryomicroscopy in situ: technology to identify molecules in cells
Recorded 15 November 2022. Chris Russo of the University of Cambridge presents "The potential of electron cryomicroscopy in situ: new technology to identify molecules in cells" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: Electron cryomicroscopy (cryoEM) of biological
From playlist 2022 Cryo-Electron Microscopy and Beyond
Cryogenic Engines | The complete physics
Let's understand the detailed working of cryogenic engines in a logical manner. Be our supporter or contributor: https://www.youtube.com/channel/UCqZQJ4600a9wIfMPbYc60OQ/join instagram : https://www.instagram.com/sabinzmathew/ Twitter : https://twitter.com/sabinsmathew Telegram : https://
From playlist Aerospace & Aeronautical
Alberto Bartesaghi - Imaging biomolecules & high-resolution single-particle cryo-electron microscopy
Recorded 13 October 2022. Alberto Bartesaghi of Duke University presents "Imaging biomolecules using high-resolution single-particle cryo-electron microscopy" at IPAM's Diffractive Imaging with Phase Retrieval Workshop. Abstract: Single-particle cryo-electron microscopy (EM) is a popular t
From playlist 2022 Diffractive Imaging with Phase Retrieval - - Computational Microscopy
Frederick Poitevin - Inference Poses for Reconstruction of 3D Molecular Volumes from Cryo-EM Images
Recorded 16 November 2022. Frederick Poitevin of SLAC National Accelerator Laboratory presents "Amortized Inference of Poses for Ab Initio Reconstruction of 3D Molecular Volumes from Experimental Cryo-EM Images" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: Cryo-electro
From playlist 2022 Cryo-Electron Microscopy and Beyond
From playlist Getting Started in Cryo-EM
Roy Lederman - Approaches for Exploring the Geometry of Molecular Conformations in Cryo-EM
Recorded 14 November 2022. Roy Lederman of Yale University Applied Mathematics presents "Approaches for Exploring the Geometry of Molecular Conformations in Cryo-EM" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: While other methods for structure determination, such as x
From playlist 2022 Cryo-Electron Microscopy and Beyond
R6. Macromolecular Electron Microscopy Applied to Fatty Acid Synthase
MIT 5.08J Biological Chemistry II, Spring 2016 View the complete course: https://ocw.mit.edu/5-08JS16 Instructor: Edward Brignole Guest speaker Edward Brignole discusses electron microscopy: what it can do, how it can be used, how it has changed in the past few years, and its applications
From playlist MIT 5.08J Biological Chemistry II, Spring 2016
A unique low temperature wavelength shifted multiple diode laser source. http://img823.imageshack.us/img823/4534/dsc0659fm.jpg http://img854.imageshack.us/img854/9815/dsc0658mc.jpg http://img194.imageshack.us/img194/5970/dsc0660gk.jpg
From playlist Lasers
Recorded 25 October 2022. Andy Minor of the University of California, Berkeley, presents at IPAM's Mathematical Advances for Multi-Dimensional Microscopy Workshop. Learn more online at: http://www.ipam.ucla.edu/programs/workshops/workshop-ii-mathematical-advances-for-multi-dimensional-micr
From playlist 2022 Mathematical Advances for Multi-Dimensional Microscopy
Peng Wang - Electron Ptychography: Emerging Computational Microscopy for Physical/Biological Science
Recorded 28 October 2022. Peng Wang of the University of Warwick presents "Electron Ptychography: An Emerging Computational Microscopy for Physical and Biological Sciences" at IPAM's Mathematical Advances for Multi-Dimensional Microscopy Workshop. Abstract: Ptychography is an emerging comp
From playlist 2022 Mathematical Advances for Multi-Dimensional Microscopy
Microscope technique brings big resolution at low temperatures
New advances in electron microscopy reveal molecular structures at resolutions useful for drug discovery. Produced by Science and the National Cancer Institute. Animation Credit: Veronica Falconieri and Sriram Subramaniam/LCB/CCR/NCI/NIH Link to article: http://scim.ag/1dYcNi0
From playlist Materials and technology
The World’s First MRI of a SINGLE Atom Is Here, and It Could Revolutionize Imaging
Magnetic resonance imaging is nothing new, but scientists were able to perform an MRI on a single atom. But how? » Subscribe to Seeker! http://bit.ly/subscribeseeker » Watch more Elements! http://bit.ly/ElementsPlaylist Scientists recently captured the smallest MRI ever while scanning a
From playlist Elements | Season 4 | Seeker
https://nationalmaglab.org/ Learn what cryogens are and why the Mag Lab couldn't function without them. The second in a 10-chapter online tour of the National High Magnetic Field Laboratory.
From playlist Virtual Tours
Day 20 2D defects and microscopy
0:00 recap of dislocation from last class 1:26 edge vs screw dislocation 4:24 dislocation motion produces deformation 4:59 2D defects, grain boundaries as series of edge dislocations 9:51 twin boundaries 10:44 stacking faults 12:35 phase boundaries and domains 14:06 optical microscopy 16:2
From playlist Introduction to Materials Science and Engineering Fall 2017
Better particle accelerators with SRF technology
The use of superconducting radio frequency (SRF) technology is a driving force in the development of particle accelerators. Scientists from around the globe are working together to develop the newest materials and techniques to improve the quality and efficiency of the SRF cavities that a
From playlist Detectors and Accelerators
Juan Carlos Idrobo - Quantum Phenomena & Electron Microscopy: New Possibilities & Limitations
Recorded 28 October 2022. Juan-Carlos Idrobo of the University of Washington presents "Into the Realms of Quantum Phenomena with Electron Microscopy: Exploring New Possibilities & Limitations" at IPAM's Mathematical Advances for Multi-Dimensional Microscopy Workshop. Abstract: Electron ene
From playlist 2022 Multi-Modal Imaging with Deep Learning and Modeling
Advancing Society Through Scientific Discovery: Catalyst: Arizona State University (ASU)
An Arizona State researcher explores the connection between capsaicin, commonly found in chili peppers, and metabolic rates. Cryo-Electron Microscopy takes near-atomic resolution snapshots of membrane proteins. A group of ASU students have found an innovative way to make trash collection m
From playlist Catalyst
Getting Started in Cryo-EM with Professor Grant Jensen
Visit the course website: http://cryo-em-course.caltech.edu Learn more about the Jensen Lab: http://www.jensenlab.caltech.edu Produced in association with Caltech Academic Media Technologies. ©2015 California Institute of Technology
From playlist Getting Started in Cryo-EM