Rhizobium Genetics and Plasmid Biology

  1. Genetic Analysis and Molecular Tools
    1. Classical Genetic Approaches
      1. Mutagenesis Techniques
        1. Chemical mutagenesis
          1. UV irradiation
            1. Transposon mutagenesis
              1. Site-directed mutagenesis
              2. Complementation Analysis
                1. Functional complementation
                  1. Plasmid rescue
                    1. Gene cloning strategies
                    2. Genetic Mapping
                      1. Linkage analysis
                        1. Physical mapping
                          1. Chromosome walking
                        2. Molecular Genetic Tools
                          1. Cloning Vectors
                            1. Broad-host-range plasmids
                              1. Shuttle vectors
                                1. Expression vectors
                                  1. Integration vectors
                                  2. Reporter Systems
                                    1. lacZ gene fusions
                                      1. gusA reporter
                                        1. Fluorescent proteins
                                          1. Luciferase systems
                                          2. Gene Expression Analysis
                                            1. Northern blotting
                                              1. RT-PCR analysis
                                                1. Quantitative PCR
                                                  1. In situ hybridization
                                                2. Modern Genomic Approaches
                                                  1. Genome Sequencing
                                                    1. Whole genome sequencing
                                                      1. Comparative genomics
                                                        1. Phylogenomic analysis
                                                          1. Pan-genome studies
                                                          2. Transcriptomic Analysis
                                                            1. RNA sequencing
                                                              1. Microarray analysis
                                                                1. Single-cell RNA-seq
                                                                  1. Temporal expression profiling
                                                                  2. Proteomic Studies
                                                                    1. Protein identification
                                                                      1. Quantitative proteomics
                                                                        1. Protein interactions
                                                                          1. Post-translational modifications
                                                                          2. Metabolomic Analysis
                                                                            1. Metabolite profiling
                                                                              1. Flux analysis
                                                                                1. Systems biology approaches
                                                                              2. Functional Genomics
                                                                                1. Gene Knockout Studies
                                                                                  1. Targeted mutagenesis
                                                                                    1. CRISPR-Cas systems
                                                                                      1. Conditional knockouts
                                                                                        1. Phenotypic analysis
                                                                                        2. Overexpression Studies
                                                                                          1. Gain-of-function analysis
                                                                                            1. Protein purification
                                                                                              1. Biochemical characterization
                                                                                              2. Protein-Protein Interactions
                                                                                                1. Two-hybrid systems
                                                                                                  1. Co-immunoprecipitation
                                                                                                    1. Cross-linking studies
                                                                                                      1. Structural analysis

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                                                                                                  7. Plasmid Evolution and Horizontal Gene Transfer

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                                                                                                  9. Applied Rhizobium Genetics