Mycology

  1. Research Methods in Mycology
    1. Collection and Isolation
      1. Field Collection
        1. Sampling Strategies
          1. Collection Techniques
            1. Field Documentation
              1. Preservation Methods
              2. Isolation Techniques
                1. Direct Isolation
                  1. Dilution Plating
                    1. Selective Isolation
                      1. Single Spore Isolation
                      2. Environmental Sampling
                        1. Soil Sampling
                          1. Water Sampling
                            1. Air Sampling
                              1. Plant Tissue Sampling
                            2. Culture Methods
                              1. Culture Media
                                1. General Purpose Media
                                  1. Selective Media
                                    1. Differential Media
                                      1. Specialized Media
                                      2. Culture Conditions
                                        1. Temperature Requirements
                                          1. pH Requirements
                                            1. Atmospheric Conditions
                                              1. Light Requirements
                                              2. Culture Maintenance
                                                1. Short-term Storage
                                                  1. Long-term Preservation
                                                    1. Cryopreservation
                                                      1. Lyophilization
                                                        1. Oil Storage
                                                          1. Silica Gel Storage
                                                      2. Microscopy and Imaging
                                                        1. Light Microscopy
                                                          1. Brightfield Microscopy
                                                            1. Phase Contrast Microscopy
                                                              1. Differential Interference Contrast
                                                                1. Fluorescence Microscopy
                                                                  1. Fluorescent Stains
                                                                    1. Fluorescent Proteins
                                                                  2. Electron Microscopy
                                                                    1. Scanning Electron Microscopy
                                                                      1. Sample Preparation
                                                                      2. Transmission Electron Microscopy
                                                                        1. Sample Preparation
                                                                      3. Specialized Imaging
                                                                        1. Confocal Microscopy
                                                                          1. Time-Lapse Microscopy
                                                                          2. Staining Techniques
                                                                            1. Vital Stains
                                                                              1. Structural Stains
                                                                                1. Lactophenol Cotton Blue
                                                                                  1. Calcofluor White
                                                                                    1. Congo Red
                                                                                    2. Functional Stains
                                                                                  2. Molecular Methods
                                                                                    1. DNA Extraction and Purification
                                                                                      1. Extraction Methods
                                                                                        1. Quality Assessment
                                                                                        2. PCR-Based Methods
                                                                                          1. Standard PCR
                                                                                            1. Real-Time PCR
                                                                                              1. Multiplex PCR
                                                                                                1. Primer Design
                                                                                                2. DNA Sequencing
                                                                                                  1. Sanger Sequencing
                                                                                                    1. Next-Generation Sequencing
                                                                                                      1. Illumina Sequencing
                                                                                                        1. PacBio Sequencing
                                                                                                          1. Oxford Nanopore
                                                                                                        2. Phylogenetic Analysis
                                                                                                          1. Sequence Alignment
                                                                                                            1. Tree Construction Methods
                                                                                                              1. Statistical Support
                                                                                                              2. Genomic Approaches
                                                                                                                1. Whole Genome Sequencing
                                                                                                                  1. Comparative Genomics
                                                                                                                    1. Transcriptomics
                                                                                                                      1. Proteomics
                                                                                                                        1. Metabolomics
                                                                                                                        2. Molecular Identification
                                                                                                                          1. DNA Barcoding
                                                                                                                            1. ITS Region
                                                                                                                              1. Other Barcode Regions
                                                                                                                              2. MALDI-TOF Mass Spectrometry
                                                                                                                            2. Experimental Design and Analysis
                                                                                                                              1. Experimental Design Principles
                                                                                                                                1. Controls
                                                                                                                                  1. Replication
                                                                                                                                    1. Randomization
                                                                                                                                    2. Statistical Analysis
                                                                                                                                      1. Descriptive Statistics
                                                                                                                                        1. Hypothesis Testing
                                                                                                                                          1. Multivariate Analysis
                                                                                                                                          2. Data Management
                                                                                                                                            1. Database Systems
                                                                                                                                              1. Data Standards
                                                                                                                                                1. Reproducibility

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                                                                                                                                            11. Plant Pathology and Phytopathogenic Fungi

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                                                                                                                                            1. Introduction to Mycology