Beta-Galactosidase Studies

  1. Catalytic Mechanism and Kinetics
    1. Enzymatic Mechanism
      1. Glycoside Hydrolase Mechanism
        1. Two-Step Hydrolysis Process
          1. Formation of Galactosyl-Enzyme Intermediate
            1. Hydrolysis of Intermediate
            2. Transition State Stabilization
              1. Role of Proton Donors and Acceptors
                1. Stereochemical Course of Reaction
                2. Enzyme Kinetics
                  1. Michaelis-Menten Model
                    1. Derivation and Assumptions
                      1. Kinetic Parameters
                        1. Km Values for Different Substrates
                          1. Vmax Determination
                            1. kcat Calculations
                            2. Catalytic Efficiency
                            3. Factors Affecting Activity
                              1. pH Dependence
                                1. Optimal pH Range
                                  1. pH-Activity Profiles
                                  2. Temperature Effects
                                    1. Optimal Temperature
                                      1. Thermal Stability
                                        1. Arrhenius Analysis
                                        2. Ionic Strength Effects
                                          1. Substrate Concentration Effects
                                        3. Enzyme Inhibition
                                          1. Competitive Inhibition
                                            1. Mechanism and Kinetics
                                              1. Common Competitive Inhibitors
                                              2. Non-competitive Inhibition
                                                1. Allosteric Sites
                                                  1. Mixed Inhibition Patterns
                                                  2. Product Inhibition
                                                    1. Irreversible Inhibition
                                                    2. Allosteric Regulation
                                                      1. Allosteric Sites
                                                        1. Cooperative Binding Effects
                                                          1. Regulatory Mechanisms

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                                                        2. Molecular Structure and Organization

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                                                        4. The lac Operon System