Beta-Galactosidase Studies

  1. Molecular Structure and Organization
    1. Primary Structure
      1. Amino Acid Composition
        1. Sequence Length and Variations
          1. Conserved Regions Across Species
            1. Signal Sequences and Processing
            2. Secondary Structure Elements
              1. Alpha Helix Content
                1. Beta Sheet Arrangements
                  1. Loop and Turn Regions
                    1. Structural Motifs
                    2. Tertiary Structure
                      1. Overall Protein Fold
                        1. Domain Architecture
                          1. TIM Barrel Domain
                            1. Beta-Galactosidase Domain
                              1. Immunoglobulin-like Domain
                              2. Structural Stability Factors
                                1. Conformational Flexibility
                                2. Quaternary Structure
                                  1. Subunit Composition
                                    1. Homotetramer Assembly
                                      1. Subunit Interfaces
                                        1. Cooperative Effects
                                          1. Assembly Mechanisms
                                          2. Active Site Architecture
                                            1. Catalytic Residues
                                              1. Glutamic Acid Residues
                                                1. Aspartic Acid Residues
                                                2. Metal Ion Coordination
                                                  1. Magnesium Ion Binding
                                                    1. Sodium Ion Binding
                                                    2. Substrate Binding Pocket
                                                      1. Conformational Changes Upon Binding

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                                                    1. Introduction to Beta-Galactosidase

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                                                    3. Catalytic Mechanism and Kinetics