Microbiome Analysis

  1. Sample Collection and Processing
    1. Pre-Collection Planning
      1. Protocol Development
        1. Equipment Preparation
          1. Contamination Prevention
            1. Chain of Custody
            2. Collection Techniques
              1. Aseptic Methods
                1. Sterile Equipment Use
                  1. Field Collection Protocols
                    1. Laboratory Collection Procedures
                    2. Sample Types and Sources
                      1. Human Samples
                        1. Fecal Samples
                          1. Skin Swabs
                            1. Oral Samples
                              1. Vaginal Samples
                                1. Urine Samples
                                  1. Blood Samples
                                  2. Environmental Samples
                                    1. Soil Samples
                                      1. Water Samples
                                        1. Air Samples
                                          1. Sediment Samples
                                          2. Animal Samples
                                            1. Gut Contents
                                              1. Skin Swabs
                                                1. Respiratory Samples
                                                2. Plant Samples
                                                  1. Rhizosphere Soil
                                                    1. Root Samples
                                                      1. Leaf Surfaces
                                                    2. Sample Preservation Methods
                                                      1. Immediate Processing
                                                        1. Freezing Protocols
                                                          1. Ultra-Low Temperature Storage
                                                            1. Liquid Nitrogen
                                                            2. Chemical Preservation
                                                              1. Ethanol Fixation
                                                                1. RNAlater Solution
                                                                  1. Formalin Fixation
                                                                  2. Lyophilization
                                                                    1. Transport Considerations
                                                                      1. Cold Chain Maintenance
                                                                        1. Shipping Protocols
                                                                          1. Documentation Requirements

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                                                                      2. Experimental Design Principles

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                                                                      4. Nucleic Acid Extraction and Quality Assessment