Drosophila and C. elegans Genetics and Development

  1. Modern Drosophila Molecular Techniques
    1. Transgenesis
      1. P-Element Transformation
        1. PhiC31 Integration
          1. Transgenic Constructs
            1. Reporter Genes
            2. GAL4-UAS System
              1. Binary Expression System
                1. GAL4 Driver Lines
                  1. UAS Responder Lines
                    1. Tissue-Specific Expression
                      1. Temporal Control
                      2. RNA Interference
                        1. RNAi Mechanism
                          1. Transgenic RNAi
                            1. Injection Methods
                              1. Off-Target Effects
                              2. CRISPR-Cas9 Genome Editing
                                1. Guide RNA Design
                                  1. Cas9 Delivery
                                    1. Homology-Directed Repair
                                      1. Non-Homologous End Joining
                                        1. Knockout Generation
                                          1. Knock-in Generation
                                          2. Optogenetics
                                            1. Channelrhodopsin
                                              1. Halorhodopsin
                                                1. Light-Activated Systems

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                                              4. Classical Drosophila Genetics

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                                              6. Drosophila Embryonic Axis Formation